Tillsammans, Ku70 och Ku80 utgör Ku-heterodimeren , vilken binder till DNA- dubbelsträngbrott ändar och krävs för den icke-homolog sammanfogning (NHEJ)

UniProt accession no. P12956. Description. General description. Lupus Ku autoantigen protein p70 (Ku70) is also known as X-

The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase. XRCC6 / Ku70 Protein LS-G28542 is a Recombinant Human XRCC6 / Ku70 produced in Wheat Germ Extract with GST tag(s). Products. Uniprot: P12956 NCBI: NM_001469 NP PA5-27538 immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 µg of Ku70 antibody (Product # PA5-27538) on 7.5% SDS-PAGE.

Ku70 uniprot

The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase. DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. We have determined that Ku70, Ku86, p300, and CREB-binding protein (CBP) are ESE-1 interacting proteins. The Ku proteins have previously been shown to bind to breaks in DNA where they function to recruit additional proteins that promote DNA repair.

Expression, Ku70 is ubiquitously Homo sapiens KU70(XRCC6) and KU80(XRCC5) protein sequences were retrieved from. UniProt and KU 70/80 beta barrel domain sequence length was Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6. Ku70 is involved in repair of G22p1, Ku70, Ku p70 less.

Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase. Ting N.S., Yu Y., Pohorelic B., Lees-Miller S.P., Beattie T.L. Maintenance of telomere integrity requires the dynamic interplay between telomerase, telomere-associated proteins and DNA repair proteins.

The kit can be used for measuring the relative amounts of Ku70/XRCC6 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Ku70/XRCC6.

PROTP12956 Ku-P70/P80 Ku P70/P80 Human Recombinant Protein KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit.

When to use UniProt Use UniProt to retrieve Ku70 autoantigen PIRSF016570:Ku80 autoantigen PIRSF Homeomorphic Family • Exactly one level • Full-length sequence similarity Ku70/80 subunits PF02735: Ku70/Ku80 beta- barrel domain Domain Superfamily • One common Pfam domain PIRSF006493: Ku DNA-binding protein, prokaryotic type PIRSF016570: Ku DNA-binding complex, Ku80 subunit PIRSF500001: IGFBP-1 pir.georgetown.edu/pirsf pir.georgetown.edu/iprolink Ku70 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ku70 Antibody (PB9520) overnight at 4°C. Not surprisingly, mutations in Ku70, Ku86, or DNA-PK(cs) result in animals that are sensitive to agents that cause DSBs and that are also immune deficient.

However, Rad3/Rad26 must have additional target(s), as cells lacking Tel1/Rad32, Rad1/Rad9/Hus1/Rad17, and Ku70 groups did not circularize chromosomes.
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Oct 6, 2020 4941, Sequence mapping, 1, The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70 The C-terminal region consists of a 5 kDa SAP domain (Ku70-SAP) which involved in DNA binding during NHEJ reaction. Expression, Ku70 is ubiquitously Homo sapiens KU70(XRCC6) and KU80(XRCC5) protein sequences were retrieved from.
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UniProt builds upon the solid foundations laid by the consortium members PIR, European Bioinformatics Institute and Swiss Institute of Bioinformatics over many years. When to use UniProt Use UniProt to retrieve Ku70 autoantigen PIRSF016570:Ku80 autoantigen PIRSF Homeomorphic Family • Exactly one level • Full-length sequence similarity

The third polypeptide, which was identified by mass spectrometry analysis, is identical to poly(ADP-ribose) polymerase-1(PARP-1), a 113-kDa enzyme that functions as a sensor of DNA damage.